Detection and analysis of leaf curl virus from Jatropha

Geminiviruses are a group of plant viruses that contain circular single stranded (ss) DNA genomes encapsidated in small twinned icosahedral capsids .The virus DNA A plus strand encodes the coat protein (CP/AV1) essential for viral transmission by whiteflies (Bemisia tabaci)[4]. Jatropha has been found to have strong molluscicidal activity and the latex to be strongly inhibitory to watermelon mosaic virus. It is also listed as a homicide, piscicide and raticide. Jatropha is considered as a poor quality fuelwood since the soft wood burns too rapidly. In Africa, Jatropha is widely planted as a “living fence” and hedgerows to protect food crops from damage by livestock and as a windbreak to prevent soil erosion moisture depletion. In Madagascar, Jatropha is used as a support plant for Vanilla. Jatropha is grown both as a vegetable and a spice crop[1]. Out of the 22 viruses infecting jatropha, mosaic and leaf curl disease are the most devastating.[5]Virus type--Single stranded DNA virus,Classification-Geminiviridae (Genus), Begomovirus (Family)[10][3] .Viral composition--Has a monopartite viral genome size of 2,750bp. Also has a satellite molecule, DNA-β satellite with a genome of 1361bp.Disease symptoms-Upward curling, vein clearing and reduced leaf size. Stunted growth and no fruit produced in severely infected plants. The virus is obligately transmitted by an insect vector, which can be the whitefly Bemisia tabaci or can be other whiteflies. This vector allows rapid and efficient propagation of the virus because it is an indiscriminate feeder. Natural conditions such as temperature of 25-35 Degrees C favor high vector population. Infected plant exhibits stunted young leaves and shoots. It grows very slowly, becomes bushy, and dwarfed. The leaf margin rolls either inward or upwards and is rather stiff with yellowish margin. Its leaves are thicker than normal, with leathery texture. The young leaves have yellowish color, cupped, thick, and rubbery. Isolate genomic DNA from jatropha plant from différent sample from diffrerent fields of lucknow. Then I have done quantitative and qualitative estimation of isolated DNA using nanodrop spectrophotometer and Agarose Gel Electrophoresis,respectively. Restriction digestion, PCR amplification of viral DNA with Begomovirus DNA-A specific primers, RAPD of a viral DNA.